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English, Jami Dyett Middle School
Genetic Engineering will explore the basic structure of the DNA
molecule, the pairing of base molecules, gene splicing, and re-
combining of DNA by chemically removing a segment of DNA from the
nucleus of a cell, and inserting it into a plasmid for reproduction.
Students will be able to identify the phosphate and sugar molecules of
the DNA strand, pair off the base molecules correctly using a paper
model, simulate the removal of DNA segments from a cell nucleus,
simulate the chemical cutting or separation along the bases of a
plasmid from a bacterium, inserting the donor strand of DNA and
inserting the DNA into the plasmid so that it can reproduce with the
new, donated characteristics provided by the inserted DNA.
Duplicated pages (both sides) from "Splice of Life" (Teachers' Guide):
Genetic Blueprint DNA Replication Splicing Life
Find The Gene Lab to Market Maze Lab to Market
1. Orally review the vocabulary listed on the Glossary page.
2. Oral reading of the Genetic Blueprint sheet and completion of
activity 2.1 to identify the phosphate and sugar molecules and
learn the base pairing rule, A to T and G to C.
3. Oral reading of activity 2.2 (Amino Acid Decoder) to learn which
DNA code for bases determines which amino acid.
4. Using a pre-cut DNA Replication sheet (activity 2.3) each group of
two students will pair off the "free" nucleotides with the unzipped
strands of DNA.
5. Oral reading of the Splicing Life sheet and completion of activity
3.1, matching the DNA strands to sites A, B, or C on the plasmid
where the restriction enzymes have chemically cut the bases to
permit the simulation of inserting the donated strands of DNA.
6. Oral reading of activity 3.2 (Find the Gene) using the base pairing
rule to construct three (3) DNA probes that will bond to the three
(3) DNA fragments given.
7. Additional activities (3.3 -Splicing Life Crossword and 4.2 - Lab
To Market Maze) can be done by simply following the given