English, Jami Dyett Middle School

Objectives: Genetic Engineering will explore the basic structure of the DNA molecule, the pairing of base molecules, gene splicing, and re- combining of DNA by chemically removing a segment of DNA from the nucleus of a cell, and inserting it into a plasmid for reproduction. Students will be able to identify the phosphate and sugar molecules of the DNA strand, pair off the base molecules correctly using a paper model, simulate the removal of DNA segments from a cell nucleus, simulate the chemical cutting or separation along the bases of a plasmid from a bacterium, inserting the donor strand of DNA and inserting the DNA into the plasmid so that it can reproduce with the new, donated characteristics provided by the inserted DNA. Apparatus Needed: Duplicated pages (both sides) from "Splice of Life" (Teachers' Guide): Genetic Blueprint DNA Replication Splicing Life Find The Gene Lab to Market Maze Lab to Market Glossary Recommended Strategy: 1. Orally review the vocabulary listed on the Glossary page. 2. Oral reading of the Genetic Blueprint sheet and completion of activity 2.1 to identify the phosphate and sugar molecules and learn the base pairing rule, A to T and G to C. 3. Oral reading of activity 2.2 (Amino Acid Decoder) to learn which DNA code for bases determines which amino acid. 4. Using a pre-cut DNA Replication sheet (activity 2.3) each group of two students will pair off the "free" nucleotides with the unzipped strands of DNA. 5. Oral reading of the Splicing Life sheet and completion of activity 3.1, matching the DNA strands to sites A, B, or C on the plasmid where the restriction enzymes have chemically cut the bases to permit the simulation of inserting the donated strands of DNA. 6. Oral reading of activity 3.2 (Find the Gene) using the base pairing rule to construct three (3) DNA probes that will bond to the three (3) DNA fragments given. 7. Additional activities (3.3 -Splicing Life Crossword and 4.2 - Lab To Market Maze) can be done by simply following the given directions.
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